Phytohormone signal transduction constitutes a fundamental regulatory framework that governs plant growth, development, and environmental adaptation by translating external stimuli into endogenous developmental programs. This laboratory course provides hands-on experience in experimentally investigating cellular-level interactions among auxin signaling regulators using multiple transient expression systems. In addition, students will perform genetic analyses of relevant mutants to deepen their understanding of plant hormone signaling dynamics and contemporary experimental approaches in plant biology.

일반생명 (General Life Sciences), 생명과학실험원리론 및 실습 (Modern Life Science Laboratory)

Week 1: Introduction to Plant Systems Biology & Primer Design

Week 2: PCR Amplification and Gel Extraction

Week 3: Restriction Enzyme Digestion, DNA Ligation, and E. coli Transformation

Week 4: Transformant Screening via Colony PCR and Cell Culture

Week 5: Plasmid Mini-Prep and Restriction Digest Confirmation

Week 6: Sequence Alignment Analysis and Maxi-Prep #1

Week 7: High-Yield Plasmid Purification (Maxi-Prep #2)

Week 8: Midterm Exam Period

Week 9: Plant Protoplast Isolation and Transfection

Week 10: In Vivo Protein-Protein Interaction Analysis via BiFC Assay

Week 11: Media Preparation, Seed Sterilization, and Sowing

Week 12: Quantitative Phenotypic and Morphological Analysis

Week 13: Investigating Subcellular Localization Dynamics via Nuclear Pore Proteins

Week 15: Final Project Presentations

Week 16: Final Exam Period